Blood Cells Study

The procedure of biological sample preparation is of critical importance. Two basic procedures are used for examination in the air. Smear method. A simple blood smear is suitable for examination. It is prepared on a slide with a standard clinical laboratory method. A 5 mkl-aliquot of blood is placed onto a slide and smeared with another slide. The smear thickness decreases along a direction of smearing. The atomic force microscope SolverP47BIO has light inverting microscope as an integral part, the latter can be used for preliminary analysis of the sample. We apply it to find the areas, where blood cells are arranged in one layer and are accessible for studying. The sample is ready to research and does not require additional manipulations. Cell sedimentation from a suspension. We use the suspension of erythrocytes in a buffer (phosphate or anyone) with pH 7.0. Cells are fixed by adding paraformaldehyde to a final concentration of 2% for 2 or more hours; then they are put into distilled water up to the concentration of 10 cell/ml. A 5 mklaliquot is placed onto a slide and dried in the air. The slide is attached to the microscope stage by doublesided stick tape.